[Objective] To prepare quercetin-loaded solid lipid nanoparticles (QT-SLN) and assess their physico-chemical and the oral absorption characteristics in mice. [Method] QT-SLN was prepared by the method of "emulsion evaporation at a high temperature and solidification at a low temperature". Stearic acid with high biocompatibility was used as lipid carrier. The optimum formulation was selected by uniform design. Its morphology was examined by transmission electron microscope (TEM) and the particle diameter distribution was decided. A UV-vis analytical method for the determination of quercetin in the nanoparticles and in the mixture of gastrointestinal contents and feces was established. [Result] QT-SLN was sphere-like and smooth. The mean diameter was 217.3 nm and the entrapment efficiency was 48.50%. The mice oral absorption of QT-SLN was better than that of quercetin solution. [Conclusion] QT-SLN can be prepared very well by the method of "emulsion evaporation at a high temperature and solidification at a low temperature" and improve the oral absorption of quercetin in mice.