Title |
Preparation of cationic gold nanoparticles and their transfection
ability into cultivated cells |
Author |
Yangde ZHANG1, Gang LIU1, Can HOU2, Jiji CHEN1, Jiwei WANG1,*, Yifeng PAN1 |
Institute
|
1. National Hepatobiliary and Enteric
Surgery Research Center, Ministry of Health, Central South University,
Changsha, Hunan 410008, P.R. China 2. Second Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China |
|
Keywords:
|
cationic gold nanoparticles; gene delivery; HepG2 cells |
| Abstract | The purpose of this research was to prepare cationic gold nanoparticles and investigate its properties and evaluate cationic gold nanoparticles as a DNA complexing agent for its efficiency in transfecting HepG2 cells. The appearances of the nanoparticles were surveyed by the SEM; the grain distribution and zeta potentials of the nanoparticles were determined with the laser grain analyzer. DNA loading efficiency of nanoparticles was examined by gel retardation assay and the gene protection effect of the nanoparticles was examined by DNase I assay. Furthermore, the transfection efficiency in vitro of the NPs was qualitatively assessed by an importation test with HepG2 cells and the result was determined by fluorescence microscope. In conclusion, cationic gold nanoparticles successfully penetrated into the HepG2 cells and fulfilled transfection of a plasmid DNA of PEGFP-C1, and cationic gold nanoparticles are worth further research as gene medicine carriers. |
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